This kit produces His-fusion proteins at high purities. The wheat germ included in this kit is a specialized wheat germ extract prepared by removing endogenous proteins as nonspecifically adsorb to His-tag affinity purification resin.
DNA-Dependent Protein Kinase Peptide Substrate is a highly specific substrate for DNA-PK, with the sequence Glu-Pro-Pro-Leu-Ser-Gln-Glu-Ala-Phe-Ala-Asp-Leu-Trp-Lys-Lys.
Recombinant full-length human PDK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2.
In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 178 to a kinase is detected by addition of a Eu-labeled anti-tag antibody.
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