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In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 178 to a kinase is detected by addition of a Eu-labeled anti-tag antibody.
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The MAO-Glo™ Assay provides a homogeneous luminescent method for measuring monoamine oxidase activity from recombinant and native sources and for testing the effects of test compounds on MAO activity.
FluoroTect Green(Lys) in vitro Translation Labeling System allows fluorescent labeling and detection of proteins synthesized in vitro. It is based on a lysine-charged tRNA labeled at the epsilon position of the lysine with the fluorophore BODIPY-FL.
DNA-Dependent Protein Kinase Peptide Substrate is a highly specific substrate for DNA-PK, with the sequence Glu-Pro-Pro-Leu-Ser-Gln-Glu-Ala-Phe-Ala-Asp-Leu-Trp-Lys-Lys.
Recombinant full-length human PDK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2.